Throughout the show, Rob is joined by his sons, Matthew and John Owen, to explore urban myths and the paranormal across America.
The 52-year-old told reporters at the launch of his programme that he had contacted what he believes were real spirits.
Rob explained that he hadn't spoken to ghosts previously, but learned how to trade messages with spirits by using an electronic device called Ovilus.
Rob Lowe claimed to have spoken to a ghost on his new show
He said that a ghost told him the name of a murder victim on the spot she died
Don't ask me how, don't ask me the science, I don't know
He said: "There's a device they use that the theory it is can translate, that the spirits can talk through this device, for lack of a better term.
"Don't ask me how, don't ask me the science, I don't know," Rob added.
The Parks and Recreation star revealed that he heard the name of a murder victim at the spot she died through Ovilus.
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"John just thinks it's randomly programmed to say words at any given time, which it could be, but how does it know to say the [right] word?
"Judge for yourself. I don't want to seem like a nut. I don't want to end my career here – too late?" Rob joked.
The Lowe Files airs in America on A&E on August 2.